152 research outputs found

    Discovery and composition of web services using artificial intelligence planning and web service modeling ontology

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    In today’s Web environment, Web services are the preferred standards-based way to realize Service Oriented Architecture (SOA) computing. A problem that has become one of the recent critical issues is automated discovery and composition of Semantic Web services. A number of approaches have been presented to solve the problem. However, most of these approaches only consider discovery or composition of Web services but not both. In this study, an effective approach called AIMO, based on Artificial Intelligence (AI) planning, Web Service Modeling Ontology (WSMO), and Semantic Web has been proposed to tackle the problem. The main purpose of this study is to investigate and develop a novel approach for automated Web service discovery and composition. In this case, a comparative evaluation of state-of-the-art approaches for Web service composition approaches has been done and the strengths and weaknesses of those approaches have been discussed. Moreover a translator for interaction between WSMO and AI-planning based on Description Logics has been proposed. In addition, some parts of AIMO architecture have been tested on a practical case study, and the results based on the experimental validation demonstrate that AIMO provides an effective and applicable solution. AIMO continues to support loose coupling paradigm of SOA by separating the discovery from the composition of Web services

    Kinematic Analysis of the Triangle-Star Robot with Telescopic Arm and Three Kinematics Chains as T-S Robot (3-PRP)

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    In this chapter, the limitations and weaknesses of the motion geometry and the workspace of Triangle-Star Robot {T-S (3-PRP)} are diagnosed after research and consideration of the issues at hand. In addition, they are offered in index form. To remove the problems with the abovementioned cases, at first, a robot with telescopic arms and a similar kinematics chain is rendered to give a kinematics analysis approach like Hartenberg-Denavit. Furthermore, in order to increase the workspace, Reuleaux Triangle-Star Robot {RT-S (3-PRP)} with kinematics chains 3-PRP and Circle-Star Robot{C-S (3-PRP)} with kinematics chains 3-PRP and a new improved structure are introduced

    Effects of solid manure particle fractionation on transport, retention, and release of Escherichia coli

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    Understanding the effect of manure particle fractionation on transport, retention, and release of bacteria plays a critical role in manure management and environmental policies that address soil and water bacterial pollution. Compared to soil particle size, there is less understanding of the importance of solid manure particle size and fractionation on bacterial fate and transport in soils. Four different cow manure particle sizes (0.25, 0.5, 1, and 2 mm) were used to investigate Escherichia coli fate in a saturated loamy sand soil. Leaching experiments were performed for up to 20 pore volumes. Preferential transport of chloride mitigated as manure particle size increased. The larger manure fractions (1 and 2 mm) showed greater heterogeneity in bacteria transport and release; smaller manure fractions (0.25 and 0.5 mm) had a greater bacteria retention with retarded release. Bacteria release was associated with transport and re-entrainment of manure particles through soil columns. The results highlighted the contribution of fine and transported particles as of primary importance for retention near the surface and transporting bacteria in soil. Similar retention shapes (i.e., exponential) for different fractions illustrated the similarity of manure source, where greater retention was observed at 0−3 cm depth for the smallest (0.25 mm) and largest (2 mm) manure fractions. The findings also highlighted the dependency of bacteria transport, retention, and release on manure physical fractionation, which should be considered in managing soil and manure practices in the field. © 2021 The Author

    Particle fractionation controls Escherichia coli release from solid manure

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    Bacteria transport through soil is a complex process particularly when the cells are released from solid manures and co-transported with particles. This study focuses on understanding of the Escherichia coli release from different particle fractions (0.25-, 0.5-, 1-, and 2-mm) of solid manure and evaluating different influent boundary conditions during cell release from manure and when a solid manure is applied to the soil. The 0.25-mm and 2-mm particle sizes resulted a greater cell release compared to 0.5-mm and 1-mm fractions (p < 0.05). The shape and magnitude of the cell release curves (CRCs) from the original manure bulk were mainly influenced by the two 0.25-mm and 2-mm fractions, respectively. The arithmetic mean for normalizing the CRCs and the time variable- based normalized CRCs for the manure-treated soil were the robust variables in evaluation of the experimental data. However, a single maximum bacteria concentration could provide the realistic dataset for the modeling process. Evaluation of the root-mean-squared-error and Akaike criterion showed that the two- and three-parametric models are recommended for simulating the cell release from solid manure in comparison with one parametric models. This study also suggests considering separate microbial release evaluations, with regards to influent concentration, for manure and manure-treated soils to propose best management practices for controlling bacteria pollution. Further research will reveal the key roles of different woody components and soluble material ratios for the various solid manures in bacteria release

    Particle fractionation controls Escherichia coli release from solid manure

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    Funding Information: This work was supported by Shahrekord University . Nasrollah Sepehrnia was supported by Alexander von Humboldt Foundation and Postdoctoral Fellowship at Leibniz University of Hannover, Germany. Publisher Copyright: © 2021 The Author(s)Peer reviewedPublisher PD

    Accelerated Start-up of a Semi-commercial Digester Tank Treating Palm Oil Mill Effluent with Sludge Seeding for Methane Production

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    The modern closed digesters are becoming more popular for treating palm oil mill effluent (POME) and are currently being installed nationwide in Malaysia to replace the conventional open lagoons and tanks treatment system. This paper describes an accelerated start-up of the 500 m semi-commercial anaerobic digester 3 treating POME and methane gas recovery for clean development mechanism (CDM) project. Results showed that by direct seeding through the transfer of the sludge from either top or bottom of the open digester tank, the start-up period was significantly shortened. The bottom seed sludge transfer led to interesting results including a 24 day start-up period, stable pH condition (pH 6.8-7.2), high COD removal efficiency (>90%), satisfactory VFA to Alk ratio (<0.3), satisfactory biogas production of nearly 1.8 kg/m /d) and methane 3 composition of 50 to 60%. The presence of high amount of methanogens in the seed sludge significantly reduced the need for a long acclimatization period and the digester could be fed with POME within less than a day after the seed sludge transfer process was completed. Close examination using scanning electron microscopy (SEM) and fluorescence in situ hybridization (FISH) revealed abundant amount of bacteria and methanogens, in particular Methanosaeta sp., in the seed sludge samples, which are very important for successful acidogenesis and methanogenesis processes

    The Effect of Mixing on Methane Production in a Semi-commercial Closed Digester Tank Treating Palm Oil Mill Effluent

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    The performance of a semi-commercial closed digester tank treating palm oil mill effluent (POME) was studied at four different mixing regimes i.e natural mixing (NM), minimal horizontal mixing (MHM), minimal horizontal and vertical mixing (MHVM) and vigorous mixing (VM). The chemical oxygen demand (COD) removal efficiency recorded satisfactory result at higher than 90% when subjected to the first three mixing regimes but reduced to the lowest of 85% when VM was applied. In the NM, MHM and MHVM experiments, the maximum total volatile fatty acids (VFA) concentration in the digester was recorded below the critical level of 1000 mg L . The MHM gave -1 the highest methane productivity at 1.4 m m d in comparison to NM at 1.0 m m d and MHVM 3 -3 -1 3 -3 -1 at 1.1 m m d. This indicates minimal mixing was required to provide good contact between 3 -3 -1 substrate and microorganisms inside the digester and to release the entrapped biogas at the bottom of the digester. The VM on the other hand was discovered to inhibit the methane production process as methane was not produced at the end of the experiment and total VFA concentration was also recorded high at 3700 mg L . The high total VFA concentration in the system may have disrupted -1 the syntrophic relationship between acidogens and methanogens and inhibited the methanogenesis

    The Effect of Higher Sludge Recycling Rate on Anaerobic Treatment of Palm Oil Mill Effluent in a Semi-Commercial Closed Digester for Renewable Energy

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    Problem statement: A 500 m3 semi-commercial closed anaerobic digester was constructed for Palm Oil Mill Effluent (POME) treatment and methane gas capture for renewable energy. During the start-up operation period, the Volatile Fatty Acids (VFA) accumulation could not be controlled and caused instability on the system.Approach: A settling tank was installed and sludge was recycled as to provide a balanced microorganisms population for the treatment of POME and methane gas production. The effect of sludge recycling rate was studied by applying Organic Loading Rates (OLR) (between 1.0 and 10.0 kgCOD m-3 day-1) at different sludge recycling rates (6, 12 and 18 m3 day-1).Results: At sludge recycling rate of 18 m3 day-1, the maximum OLR was 10.0 kgCOD m-3 day-1 with biogas and methane productivity of 1.5 and 0.9 m3 m-3 day-1, respectively. By increasing the sludge recycling rate the VFA concentration was controlled below its inhibitory limit (1000 mg L-1) and the COD removal efficiency recorded was above 95% which indicated good treatment performance for the digester. Two methanogens species (Methanosarcina sp. and Methanosaeta concilii) had been identified from sludge samples obtained from the digester and recycled stream.Conclusion: By increasing the sludge recycling rate upon higher application of OLR, the treatment process was kept stable with high COD removal efficiency. The biogas and methane productivity were initially improved but reduced once OLR and recycling rate were increased to 10.0 kg COD m3 day-1 and 18 m3 day-1 respectively

    Polyhydroxyalkanoate production from anaerobically treated palm oil mill effluent by new bacterial strain Comamonas sp. EB172

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    A new isolate designated as strain EB172 was isolated from a digester treating palm oil mill effluent and was investigated by polyphasic taxonomic approach. The cells were rod-shaped, Gram-negative, non-pigmented, non-spore-forming and non-fermentative. Phylogenetic analysis using the 16S rRNA gene sequence showed that the strain clustered with the genus Comamonas. Its closest neighbours were the type strains Comamonas terrigena (96.8%), Comamonas koreensis (93.4%), Comamonas composti (92.9%), and Comamonas kerstersii (91.1%). The ability of the strain EB172 to produce polyhydroxyalkanoates (PHA) when supplied with organic acids made this bacterium unique among Comamonas species. The bacterial strain was clearly distinguished from all of the existing strains by phylogenetic analysis, fatty acid composition and a range of physiological and biochemical characteristics. The G+C content of the genomic DNA was 59.1 mol%. The strain showed good growth in acetic, propionic and n-butyric acids. Comamonas sp. EB172 produced 9.8 g/l of cell dry weight and accumulated 59 (wt%) of PHAs when supplemented with mixed organic acids from anaerobically treated palm oil mill effluent. It is evident from the genotypic, phenotypic data and ability to produce PHAs that strain EB172 represents a new strain in the genus Comamonas (GeneBank accession no. EU847238)

    Comparative study of methods for extraction and purification of environmental DNA from high-strength wastewater sludge

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    DNA extraction from wastewater sludge (COD 50000 and BOD 25000 mg/l) was conducted using nine different methods normally used for environmental samples including a procedure used in this study and the results obtained were compared. The quality of the differently extracted DNAs was subsequently assessed by measuring humic acid concentration, cell lysis efficiency, polymerase chain reaction (PCR) amplification of methanogenic and eubacterial 16S rDNA. The protocol developed in this study was further evaluated by extracting DNA from various high-strength wastewater sludge samples, denaturing gradient gel electrophoresis (DGGE) and fluorescent in situ hybridization (FISH) analyses. The results revealed that great differences existed among the nine procedures and only a few produced satisfactory results when applied to high-strength wastewater sludge. Thermal shock alone was shown inefficient to disrupt the methanogenic cell wall to release the DNA. The method presented in this study (Procedure 9) is generally recommended because of the low concentration of contaminants and its high efficiency despite its simplicity.Key words: High-strength wastewater sludge, DNA extraction, environmental samples, humic acids, denaturing gradient gel electrophoresis, fluorescent in situ hybridizatio
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